Abstract: The stage of higher education is an important period for college students to grow up physically and mentally and form their health literacy. Setting up college student health education course is the best way to improve their health literacy. In recent years, the Ministry of education has issued a series of documents, which have new requirements on the teaching content and teaching mode of College Student health education. In order to further stimulate students’ interest in learning and improve the teaching quality, we explore the application of special subject teaching method,multimedia teaching method and practical teaching method based on PBL concept, which can improve the teaching quality and students’ health literacy.